Monitoring lectin interactions with carbohydrates.

Sophie De Bentzmann (1) , Annabelle Varrot (2) , Anne Imberty (2)

1 LISM - Laboratoire d'ingénierie des systèmes macromoléculaires
2 CERMAV - Centre de Recherches sur les Macromolécules Végétales

Protein-carbohydrate interactions are often involved in the first step of infection and Pseudomonas aeruginosa produces several proteins that are able to bind specifically to glycan epitopes present on host epithelia. The experimental approaches for studying protein-carbohydrate interaction have been inspired, with some adaptations, from those commonly used for protein-protein or protein-ligand interactions. A range of methods are described herein for detecting lectin activity, screening for monosaccharide or oligosaccharide specificity, determining the affinity of binding together with thermodynamics and kinetics parameters, and producing crystal of lectin-carbohydrate complexes for further structural studies.

Domaines: Biochimie, Biologie Moléculaire

Sophie De Bentzmann, Annabelle Varrot, Anne Imberty. Monitoring lectin interactions with carbohydrates.. Methods in Molecular Biology, 2014, 1149, pp.403--14. ⟨10.1007/978-1-4939-0473-0_32⟩. ⟨hal-01458201⟩