Performance of MALDI-TOF MS platforms for fungal identification

Carole Cassagne (1, 2) , Anne-Cécile Normand (2) , Coralie L'Ollivier (3) , Stephane Ranque (4) , Renaud Piarroux (1)

1 AMU - Aix Marseille Université
2 IP-TPT - Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques
3 CHU Dijon
4 URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes

Matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-TOF MS) is increasingly used by clinical microbiology laboratories to cope with the need for rapid, cost-effective and accurate identification of microorganisms. Several research teams have recently succeed in identifying moulds using MALDI-TOF MS, which was first adapted to bacteria, then to yeast identification. Since 2004, different commercial firms have released several ready-to-use MALDI-TOF MS platforms. This review describes the similarities and differences between the commercially available systems. In two parts, we first describe and compare the preprocessing and identification steps between the platforms and then compare the identification efficacy of yeast, moulds and dermatophytes species.

Domaines: Maladies infectieuses

Carole Cassagne, Anne-Cécile Normand, Coralie L'Ollivier, Stephane Ranque, Renaud Piarroux. Performance of MALDI-TOF MS platforms for fungal identification. Mycoses, 2016, 59 (11), pp.678-690. ⟨10.1111/myc.12506⟩. ⟨hal-01453298⟩