Anti-heparanase activity of ultra-low-molecular-weight heparin produced by physicochemical depolymerization

Abstract : Heparanase is an endo-β-D-glucuronidase that plays an important role in cancer progression, in particular during tumor angiogenesis and metastasis. Inhibiting this enzyme is considered as one of the most promising approaches in cancer therapy. Heparin is a complex glycoaminoglycan known as a strong inhibitor of heparanase. It is primarily used in clinical practice for its anticoagulant activities, which may not be compatible with its use as anti-angiogenic agent. In this study, we described the production of ultra-low-molecular-weight heparins (ULMWH) by a physicochemical method that consists in a hydrogen peroxide-catalyzed radical hydrolysis assisted by ultrasonic waves. We assessed the structural characteristics, anticoagulant and anti-heparanase activities of the obtained heparin derivatives and compared them with three commercial low-molecular-weight heparins (LMWH), glycol-split non-anticoagulant heparins and heparins produced by enzymatic methods. ULMWH generated by the physicochemical method were characterized by high anti-heparanase and moderate anticoagulant activities. These heparin derivatives might be potential candidates for cancer therapy when a compromise is needed between anti-heparanase and anticoagulant activities.
Type de document :
Article dans une revue
Carbohydrate Polymers, Elsevier, 2016, 〈10.1016/j.carbpol.2015.08.041〉
Liste complète des métadonnées

https://hal.archives-ouvertes.fr/hal-01447822
Contributeur : Viviane Biou <>
Soumis le : vendredi 27 janvier 2017 - 12:01:43
Dernière modification le : jeudi 9 février 2017 - 16:58:54

Identifiants

Collections

Citation

Oussama Achour, Nicolas Poupard, Bridiau Nicolas, Stéphanie Bordenave-Juchereau, Frédéric Sannier, et al.. Anti-heparanase activity of ultra-low-molecular-weight heparin produced by physicochemical depolymerization . Carbohydrate Polymers, Elsevier, 2016, 〈10.1016/j.carbpol.2015.08.041〉. 〈hal-01447822〉

Partager

Métriques

Consultations de la notice

29