Protein corona formation for nanomaterials and proteins of a similar size: hard or soft corona? - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nanoscale Année : 2013

Protein corona formation for nanomaterials and proteins of a similar size: hard or soft corona?

Résumé

Nanoparticles (NPs) entering a biological fluid undergo surface modification due to dynamic, physicochemical interactions with biological components, especially proteins. In this work we used complementary bio-physico-chemical approaches to characterize the effects of interactions between CeO2 NPs, immunoglobulins (IgGs) and bovine serum albumin (BSA) of a similar size on protein structural evolution as well as formation of (hetero-) aggregates. Using circular dichroism we showed that IgGs and BSA underwent significant structural changes after interaction with NPs. The NPs and protein-NPs were observed after size exclusion chromatography, highlighting the fact that few aggregates were stable enough to pass this mild separation step. X-ray absorption spectroscopy suggested that the surface chemistry of NPs was not affected by these proteins, also implying weak interactions. Competitive experiments revealed that, while the serum was more concentrated for BSA, IgG-NP aggregates were more stable. Altogether, our results indicate that, under our experimental conditions, the formation of a ``protein corona'' is an unstable and reversible mechanism. This indicates that, when NPs and proteins are similar in size, the adsorption concept (i.e. protein corona concept) cannot be applied to model the NP-protein interaction, and a heteroaggregation model is more appropriate.
Fichier non déposé

Dates et versions

hal-01426275 , version 1 (04-01-2017)

Identifiants

Citer

Wei Liu, Jérôme Rose, Sophie Plantevin, Melanie Auffan, Jean-Yves Bottero, et al.. Protein corona formation for nanomaterials and proteins of a similar size: hard or soft corona?. Nanoscale, 2013, 5 (4), pp.1658-1668. ⟨10.1039/c2nr33611a⟩. ⟨hal-01426275⟩
205 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More