Bacterial Proliferation: Keep Dividing and Don't Mind the Gap

Luisa Laureti (1) , Julien Demol (1) , Robert P Fuchs (1) , Vincent Pagès (1)

1 CRCM - Centre de Recherche en Cancérologie de Marseille

DNA Damage Tolerance (DDT) mechanisms help dealing with unrepaired DNA lesions that block replication and challenge genome integrity. Previous in vitro studies showed that the bacterial replicase is able to re-prime downstream of a DNA lesion, leaving behind a single-stranded DNA gap. The question remains of what happens to this gap in vivo. Following the insertion of a single lesion in the chromosome of a living cell, we showed that this gap is mostly filled in by Homology Directed Gap Repair in a RecA dependent manner. When cells fail to repair this gap, or when homologous recombination is impaired, cells are still able to divide, leading to the loss of the damaged chromatid, suggesting that bacteria lack a stringent cell division checkpoint mechanism. Hence, at the expense of losing one chromatid, cell survival and proliferation are ensured. Author Summary DNA Damage Tolerance (DDT) mechanisms help dealing with unrepaired DNA lesions that block replication, thus challenging genome integrity. Two DDT mechanisms have previously been described: error prone Translesion Synthesis operated by specialized DNA polymerases and error free bypass that uses the information of the sister chromatid to bypass the lesion. In this work, we set up a novel genetic system that allows to insert a single DNA blocking lesion in the chromosome of a living cell and to visualize the exchange of genetic information between the undamaged and the damaged strand. Using this system, we showed in vivo that the replication fork is able to re-prime downstream of the lesion, leaving a gap. This gap is mostly filled in by the error free pathway through the RecA homologous recombination mechanism. We show that when the gap is left unre-paired, cells are still able to divide by losing the damaged chromatid, which evidences the lack of a stringent cell division checkpoint system.

Domaines: Biologie moléculaire

Luisa Laureti, Julien Demol, Robert P Fuchs, Vincent Pagès. Bacterial Proliferation: Keep Dividing and Don't Mind the Gap. PLoS Genetics, 2015, 11, ⟨10.1371/journal.pgen.1005757⟩. ⟨hal-01426227⟩