Skip to Main content Skip to Navigation
Journal articles

Enzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging

Abstract : Invivo investigations of enzymatic processes using non-invasive approaches are a long-lasting challenge. Recently, we showed that Overhauser-enhanced MRI is suitable to such a purpose. A -phosphorylated nitroxide substrate prototype exhibiting keto-enol equilibrium upon enzymatic activity has been prepared. Upon enzymatic hydrolysis, a large variation of the phosphorus hyperfine coupling constant (a(P)=4G) was observed. The enzymatic activities of several enzymes were conveniently monitored by electronic paramagnetic resonance (EPR). Using a 0.2T MRI machine, invitro and invivo OMRI experiments were successfully performed, affording a 1200% enhanced MRI signal invitro, and a 600% enhanced signal invivo. These results highlight the enhanced imaging potential of these nitroxides upon specific enzymatic substrate-to-product conversion.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01413652
Contributor : valerie Roubaud Connect in order to contact the contributor
Submitted on : Saturday, December 10, 2016 - 3:09:13 PM
Last modification on : Monday, March 28, 2022 - 10:34:02 AM

Links full text

Identifiers

Collections

Citation

Gérard Audran, Lionel Bosco, Paul Brémond, Jean-Michel Franconi, Neha Koonjoo, et al.. Enzymatically Shifting Nitroxides for EPR Spectroscopy and Overhauser-Enhanced Magnetic Resonance Imaging. Angewandte Chemie International Edition, Wiley-VCH Verlag, 2015, 54 (45), pp.13379 - 13384. ⟨10.1002/anie.201506267⟩. ⟨hal-01413652⟩

Share

Metrics

Record views

123