Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation

Shahnoo Khoyratty (1, 2, 3) , Joëlle Dupont (4) , Sandrine Lacoste (4) , Tony Lionel Palama (5, 3, 2) , Young Hae Choi (1) , Hye Kyong Kim (1) , Bertrand Payet (6) , Michel Grisoni (7) , Mireille Fouillaud (6) , Robert Verpoorte (1) , Hippolyte Kodja (3)

1 Natural Products Laboratory
2 CYROI - Cyclotron Réunion Océan Indien
3 UMR PVBMT - Peuplements végétaux et bioagresseurs en milieu tropical
4 Département Systématique et Évolution
5 ISA - Institut des Sciences Analytiques
6 LCSNSA - Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments
7 3P, IBiSA - Pôle de Protection des Plantes

Background: The objective of the work was to characterize fungal endophytes from aerial parts of Vanilla planifolia. Also, to establish their biotransformation abilities of flavor-related metabolites. This was done in order to find a potential role of endophytes on vanilla flavors. Results: Twenty three MOTUs were obtained, representing 6 fungal classes. Fungi from green pods were cultured on mature green pod based media for 30 days followed by 1 H NMR and HPLC-DAD analysis. All fungi from pods consumed metabolized vanilla flavor phenolics. Though Fusarium proliferatum was recovered more often (37.6 % of the isolates), it is Pestalotiopsis microspora (3.0 %) that increased the absolute amounts (quantified by 1 H NMR in μmol/g DW green pods) of vanillin (37.0 × 10 −3), vanillyl alcohol (100.0 × 10 −3), vanillic acid (9.2 × 10 −3) and p-hydroxybenzoic acid (87.9 × 10 −3) by significant amounts.

Mots clés: Endophytes; Distribution; Diversity; Biotransformation; Vanilla; Interaction

Domaines: Biologie végétale

Shahnoo Khoyratty, Joëlle Dupont, Sandrine Lacoste, Tony Lionel Palama, Young Hae Choi, et al.. Fungal endophytes of Vanilla planifolia across Réunion Island: isolation, distribution and biotransformation. BMC Plant Biology, 2015, 10, pp.301 - 301. ⟨10.1186/s12870-015-0522-5⟩. ⟨hal-01397514⟩