A Fluorescent Polymer Probe with High Selectivity toward Vascular Endothelial Cells for and beyond Noninvasive Two-Photon Intravital Imaging of Brain Vasculature

B. Mettra (1) , F. Appaix (2) , J. Olesiak-Banska (3) , T. Le Bahers (1) , A. Leung (1) , K. Matczyszyn (3) , M. Samoc (3) , B. van Der Sanden (4) , C. Monnereau (1) , C. Andraud (1)

1 LC - Laboratoire de Chimie - UMR5182
2 GIN - [GIN] Grenoble Institut des Neurosciences
3 Institute of Physical and Theoretical Chemistry
4 SCLIN - Clinatec - Centre de recherche biomédicale Edmond J.Safra

A chromophore-engineering strategy that relies on the introduction of a ground-state distortion in a quadrupolar chromophore was used to obtain a quasi-quadrupolar chromophore with red emission and large two-photon absorption (2PA) cross-section in polar solvents. This molecule was functionalized with water-solubilizing polymer chains. It constitutes not only a remarkable contrast agent for intravital two-photon microscopy of the functional cerebral vasculature in a minimally invasive configuration but presents intriguing endothelial staining ability that makes it a valuable probe for premortem histological staining.

Domaines: Catalyse; Matériaux; Chimie organique; Chimie inorganique

B. Mettra, F. Appaix, J. Olesiak-Banska, T. Le Bahers, A. Leung, et al.. A Fluorescent Polymer Probe with High Selectivity toward Vascular Endothelial Cells for and beyond Noninvasive Two-Photon Intravital Imaging of Brain Vasculature. ACS Applied Materials & Interfaces, 2016, 8 (27), pp.17047 - 17059. ⟨10.1021/acsami.6b02936⟩. ⟨hal-01378703⟩