Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients

Abstract : The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes. (C) 2013 Elsevier Ltd. All rights reserved.
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Submitted on : Wednesday, September 28, 2016 - 3:07:29 PM
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Patricia Costaglioli, Christophe Barthe, Michaël Fayon, Naik Christoflour, Stéphanie Bui, et al.. Selection of Pseudomonas aeruginosa reference genes for RT-qPCR analysis from sputum of cystic fibrosis patients. Molecular and Cellular Probes, Elsevier, 2014, 28 (1), pp.10-12. ⟨10.1016/j.mcp.2013.09.003 ⟩. ⟨hal-01373321⟩

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