Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy

Susann Spindler (1, 2) , Jens Ehrig (1, 3) , Katharina König (1, 2) , Tristan Nowak (4) , Marek Piliarik (1, 5) , Hannah E. Stein (2) , Richard W. Taylor (1) , Elisabeth Garanger (6, 7, 8) , Sébastien Lecommandoux (6, 8) , Isabel D. Alves (9) , Vahid Sandoghdar (1, 2)

1 Max Planck Institute for the Science of Light
2 FAU - Friedrich-Alexander Universität Erlangen-Nürnberg = University of Erlangen-Nuremberg
3 B CUBE-Ctr Mol Bioengn, TUD Dresden
4 OICE - Optical Imaging Center Erlangen
5 UFE / CAS - Institute of Photonics and Electronics of the Czech Academy of Sciences
6 LCPO - Laboratoire de Chimie des Polymères Organiques
7 IECB - Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie
8 Team 3 LCPO : Polymer Self-Assembly & Life Sciences
9 CBMN - Chimie et Biologie des Membranes et des Nanoobjets

Microscopy based on the interferometric detection of light scattered from nanoparticles (iSCAT) was introduced in our laboratory more than a decade ago. In this work, we present various capabilities of iSCAT for biological studies by discussing a selection of our recent results. In particular, we show tracking of lipid molecules in supported lipid bilayers (SLBs), tracking of gold nanoparticles with diameters as small as 5 nm and at frame rates close to 1 MHz, 3D tracking of Tat peptide-coated nanoparticles on giant unilamellar vesicles (GUVs), imaging the formation of lipid bilayers, sensing single unlabelled proteins and tracking their motion under electric fields, as well as challenges of studying live cell membranes. These studies set the ground for future quantitative research on dynamic biophysical processes at the nanometer scale.

Mots clés: single molecule detection; protein detection; single protein electrophoresis; lipid diffusion; Electric fields; Cytology; single-particle tracking; Interferometric detection; Molecules; Light scattering; Interferometry; Nanoparticles; Lipid bilayers; Proteins; Electromagnetic wave scattering; Electrophoresis

Domaines: Chimie analytique; Chimie-Physique [physics.chem-ph]; Biophysique [physics.bio-ph]; Optique [physics.optics]

Susann Spindler, Jens Ehrig, Katharina König, Tristan Nowak, Marek Piliarik, et al.. Visualization of lipids and proteins at high spatial and temporal resolution via interferometric scattering (iSCAT) microscopy. Journal of Physics D: Applied Physics, 2016, 49 (27), pp.274002. ⟨10.1088/0022-3727/49/27/274002⟩. ⟨hal-01372499⟩