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Gold Nanoparticles Grafted by Reduced Glutathione With Thiol Function Preservation

Abstract : Gold nanoparticles (AuNP) are one of the most widely applied nanomaterials for biomedical purposes due to their ease of preparation,well-defined structure and simple surface functionalization.Herein, a method to anchor reduced glutathione (GSH, i.e. an important antioxidant tripeptide in all living organisms) onto AuNP surface with preservation of the GSH thiol function was reported. Using AuNP capped with dihydrolipoic acid (AuNP@DHLA) as starting material, GSH was anchored by an N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS)-mediated coupling between the carboxylate group of DHLA and the amine function of GSH to generate AuNP@DHLA-(GSH)n. Yields of 7.5 ±1.2 μMof thiol per nM of AuNPwere obtained. These particles showed a markedly enhanced reductive capacity compared to AuNP@DHLA and free GSH evaluated by two assays commonly used in evaluation of antioxidant activities, i.e. 2,2′-azino-bis(3-ethylbenzothiazoline- 6-sulphonic acid) and Ferric ion Reducing Antioxidant Power assays, and by direct potentiometry.
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Contributor : Raphaël Schneider <>
Submitted on : Monday, September 26, 2016 - 6:39:28 PM
Last modification on : Thursday, October 3, 2019 - 4:42:15 PM

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Ming Luo, Ariane Boudier, Igor Clarot, Philippe Maincent, Raphael Schneider, et al.. Gold Nanoparticles Grafted by Reduced Glutathione With Thiol Function Preservation. Colloids and Interface Science Communications, Elsevier, 2016, 14, pp.8-12. ⟨10.1016/j.colcom.2016.07.002⟩. ⟨hal-01372065⟩



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