Enzyme-assisted extraction (EAE) for the production of antiviral and antioxidant extracts from the green seaweed Ulva armoricana (Ulvales, Ulvophyceae)
Résumé
In order to develop innovative methods for the production of bioactive extracts with potential health benefits,
enzyme-assisted extraction has been applied for the bioconversion of Ulva armoricana (Ulvales, Ulvophyceae).
The extraction yields, chemical composition, the antioxidant and antiviral activities were determined to assess
the efficiency of six commercial enzymatic preparations. Endo-protease treatments significantly increased the
extraction yields compared to the control. The organic matter, neutral sugar and protein contentswere increased
in all extracts compared to an extractionwith water, up to 2.0-fold, 2.7-fold and 1.75-fold, respectively. The samples
prepared by amultiple-mix of glycosyl-hydrolases and an exo-β-1,3(4)-glucanase revealed activities against
herpes simplex virus type-1 at the median effective concentration (EC50) of 373.0±20.7 and 320.9±33.6 μg/ml,
respectively. These activities were correlated to high amounts of rhamnose, uronic acids and sulfate groups
which are the main constituents of ulvans. Free radical scavenging capacity at mediuminhibition concentrations
(IC50) of 1.8 and 12.5 mg/mlwas shown for the extracts producedwith endo-protease treatments and 6.0 mg/ml
for the sample resulting from the extraction with the multiple-mix of glycosyl-hydrolases. This study reports the
antiviral and antioxidant activities of Ulva armoricana extracts produced by enzyme-assisted extraction.