Design and characterization of red fluorogenic push–pull chromophores holding great potential for bioimaging and biosensing

Chenge Li (1, 2) , Marie-Aude Plamont (2, 1) , Isabelle Aujard (2, 1) , Thomas Le Saux (2, 1) , Ludovic Jullien (2, 1, 3) , Arnaud Gautier (2, 1)

1 Département de Chimie - ENS Paris
2 UPMC - Université Pierre et Marie Curie - Paris 6
3 PASTEUR - Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640)

Fluorogenic chromophores have been used recently for fluorescence reporting and biosensing. Their ability to turn on upon specific interaction with a given target has been exploited in particular for the design of fluorogen-based reporters enabling biomolecule labeling and imaging. In this paper, we report the development and exhaustive characterization of a new family of red fluorogenic push–pull chromophores, holding great potential for the development of fluorogen-based reporters or intracellular fluorogenic markers. The proposed methodology is generic and should find general applicability in the discovery of new fluorogenic dyes suitable for the design of fluorogen-based reporters and biosensors.

Domaines: Chimie

Chenge Li, Marie-Aude Plamont, Isabelle Aujard, Thomas Le Saux, Ludovic Jullien, et al.. Design and characterization of red fluorogenic push–pull chromophores holding great potential for bioimaging and biosensing. Organic & Biomolecular Chemistry, 2016, 14, pp.9253-9261. ⟨10.1039/C6OB01612J⟩. ⟨hal-01366661⟩