4D holographic microscopy of zebrafish larvae microcirculation

Abstract : An original technique that combines digital holography, dual illumination of the sample and cleaning algorithm 3D reconstruction is proposed. It uses a standard transmission microscopy setup coupled with a digital holography detection. The technique is 4D, since it allows to determine, at each time step, the 3D locations (x, y, z) of many moving objects that scatter the dual illumination beam. The technique has been validated by imaging the microcirculation of blood in a fish larvae sample (the moving objects are thus red blood cells RBCs). Videos showing in 4D the moving RBCs superimposed with the perfused blood vessels are obtained.
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https://hal.archives-ouvertes.fr/hal-01363227
Contributor : Michel Gross <>
Submitted on : Friday, September 9, 2016 - 2:51:22 PM
Last modification on : Tuesday, November 5, 2019 - 5:14:35 PM
Long-term archiving on: Saturday, December 10, 2016 - 1:09:42 PM

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Dario Donnarumma, Alexey Brodoline, Daniel Alexandre, Michel Gross. 4D holographic microscopy of zebrafish larvae microcirculation. Optics Express, Optical Society of America, 2016, 24 (23), pp.26887-26900. ⟨10.1364/OE.24.026887⟩. ⟨hal-01363227⟩

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