Poly(2-oxazoline)-Based Nanogels as Biocompatible Pseudopolypeptide Nanoparticles

Camille Legros (1, 2) , Anne-Laure Wirotius (1) , Marie-Claire de Pauw-Gillet (3) , Kam Chiu Tam (2) , Daniel Taton (1, 4) , Sébastien Lecommandoux (1, 5)

1 LCPO - Laboratoire de Chimie des Polymères Organiques
2 Institute for Nanotechnology [Waterloo]
3 GIGA-R - Laboratory of Mammalian Cell Culture
4 Team 1 LCPO : Polymerization Catalyses & Engineering
5 Team 3 LCPO : Polymer Self-Assembly & Life Sciences

Hydrophilic nanogels based on partially hydrolyzed poly(2-ethyl-2-oxazoline) were synthesized in dilute aqueous media in the presence of 1,6-hexanediol diglycidyl ether as a cross-linker. Nanogel formation was monitored by DLS and HSQC NMR spectroscopy, and the final nano-objects were characterized by DLS, TEM, AFM, and NanoSight analyses. Nanogels with a hydrodynamic radius of 78 nm exhibiting a slight positive surface charge were obtained. MTS assays (cell metabolic activity test) evidenced that nanogels were nontoxic in the investigated concentration range (i.e., 0.1 to 400 mu g/mL) and that no specific interaction with bovine serum albumin was observed.

Mots clés: POLY(2-ETHYL-2-OXAZOLINE); GENE DELIVERY; IN-VITRO; DRUG-DELIVERY; PROTEIN ADSORPTION; POLYMERIC NANOPARTICLES; BIOMEDICAL APPLICATIONS; POLY(ETHYLENE GLYCOL); AGGREGATION BEHAVIOR; BLOCK-COPOLYMERS

Domaines: Polymères

Camille Legros, Anne-Laure Wirotius, Marie-Claire de Pauw-Gillet, Kam Chiu Tam, Daniel Taton, et al.. Poly(2-oxazoline)-Based Nanogels as Biocompatible Pseudopolypeptide Nanoparticles . Biomacromolecules, 2015, 16 (1), pp.183-191. ⟨10.1021/bm501393q⟩. ⟨hal-01361917⟩