PSF engineering in multifocus microscopy for increased depth volumetric imaging

Bassam Hajj (1, 2) , Mohamed El Beheiry (1, 2) , Maxime Dahan (1, 2)

1 PCC - Physico-Chimie-Curie
2 Janelia Farm Research Campus

Imaging and localizing single molecules with high accuracy in a 3D volume is a challenging task. Here we combine multifocal microscopy, a recently developed volumetric imaging technique, with point spread function engineering to achieve an increased depth for single molecule imaging. Applications in 3D single molecule localization-based super-resolution imaging is shown over an axial depth of 4 µm as well as for the tracking of diffusing beads in a fluid environment over 8 µm.

Mots clés: Three-dimensional microscopy; Three dimensional image processing; Super-resolution

Domaines: Imagerie; Instrumentations et Détecteurs [physics.ins-det]

Bassam Hajj, Mohamed El Beheiry, Maxime Dahan. PSF engineering in multifocus microscopy for increased depth volumetric imaging. Biomedical optics express, 2016, 7 (3), pp.726-731. ⟨10.1364/BOE.7.000726⟩. ⟨hal-01306777⟩