Prevalence of the pathogen Aphanomyces astaci in freshwater crayfish populations in Croatia

I Maguire M Jelić G Klobučar M Delpy 1 C Delaunay 1 F Grandjean 1
1 EES - Ecologie, Evolution, Symbiose
EBI - Ecologie et biologie des interactions
Abstract : The Oomycete Aphanomyces astaci is an obligate crayfish parasite that co-evolved with American crayfish species, and they therefore generally live in a balanced relationship. On the contrary, European native crayfish are highly susceptible to A. astaci, and infestation with it causes development of the lethal disease termed crayfish plague. Until now, 5 A. astaci strains have been described from the freshwater crayfish present in Europe. In this study we aimed to investigate the occurrence of the pathogen A. astaci in Croatian native and non-native crayfish populations, as well as to genotype established strains using microsatellite markers and obtain information on the pathogens epidemiology. Our results showed that the pathogen is widespread in both native and non-native crayfish populations. Agent level, when positive, in non-native crayfish was generally low; in native species it was higher. Genotyping from microsatellites proved the presence of the B (Ps) strain in non-native species (Pacifastacus leniusculus), while the A (As) strain was detected from viable native species (Astacus astacus and Austropotamobius torrentium) that are distributed in areas lacking non-native crayfish. The genotype from A. torrentium differed from a typical A (As) by 1 allele. Strain B (Ps) was identified in native Astacus leptodactylus from the population that co-occurs with P. leniuscuslus. Interestingly, in 1 A. leptodactylus population both A (As) and B (Ps) strains were present.
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Submitted on : Friday, February 12, 2016 - 3:15:02 PM
Last modification on : Wednesday, November 6, 2019 - 3:40:04 PM

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I Maguire, M Jelić, G Klobučar, M Delpy, C Delaunay, et al.. Prevalence of the pathogen Aphanomyces astaci in freshwater crayfish populations in Croatia. Diseases of Aquatic Organisms, Inter Research, 2016, 118 (1), pp.45-53. ⟨10.3354/dao02955⟩. ⟨hal-01273507⟩

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