UV-C radiation modifies the ripening and accumulation of ethylene response factor (ERF) transcripts in tomato fruit

Joseana Severo (1) , Aline Tiecher (2) , Jullien Pirrello (3) , Farid Regad (3) , Alain Latché (3) , Jean-Claude Pech (3) , Mondher Bouzayen (3) , César Valmor Rombaldi (4)

1 Instituto Federal Farroupilha
2 Universidade Federal do Pampa
3 GBF - Génomique et Biotechnologie des Fruits
4 Universidade de Pelotas

Ultraviolet-C (UV-C) radiation is used as a postharvest treatment to prolong the shelf life of fruit. However, this stressful process may also affect ethylene production and, consequently, the expression of genes encoding ethylene response factors (ERFs). To test this hypothesis, MicroTom tomatoes harvested at the breaker stage were subjected to: 1 – application of 3.7 kJ m-2 UV-C radiation, 2 – application of 2 µLL-1 1-methylcyclopropene (1-MCP) followed by UV-C radiation; and 3 – without 1-MCP or UV-C (control treatment). After treatment all fruit were stored for 12 d at 21 +/- 2C° and 80 +/- 5% relative humidity (RH). Although UV-C radiation increased ACC oxidase transcripts and stimulated ethylene production, the ripening evolution was delayed. Fruit treated with UV-C showed lower accumulation of lycopene, b-carotene, lutein + zeaxanthin and d-tocopherol; but retained higher levels of chlorogenic acid, r-coumaric acid and quercetin after 6 d. Additionally, UV-C treated fruit had higher contents of polyamines (putrescine and spermidine). Among the 14 ERFs studied, 11 (Sl-ERF A.1, Sl-ERF A.3,Sl-ERF B.1, Sl-ERF B.2, Sl-ERF B.3, Sl-ERF C.6, Sl-ERF D.1, Sl-ERF D.3, Sl-ERF E.1, Sl-ERF F.5, Sl-ERF G.2) exhibited increased transcript accumulation, 2 ERFs (Sl-ERF E.2 and Sl-ERF E.4) showed decreased transcript accumulation and only 1 ERF (Sl-ERF E.3) was not significantly affected by UV-C treatment. As expected, the transcript profiles of 1-MCP and/or UV-C-treated tomatoes demonstrate that ethylene plays an important role in the expression of ERFs. The delay in fruit ripening may be caused by the activation of ERFs that could act as regulators of metabolic pathways during ripening. However, this hypothesis needs to be better tested. In conclusion, a relationship has been established between UV-C treatment and ripening delay, correlated to changes in 13 ERF transcripts evaluated during postharvest treatment.

Mots clés: Ripening; Abiotic stress; Senescence; Solanumlycopersicum; Ultraviolet C; 1-Methylcyclopropene

Domaines: Ingénierie des aliments; Agronomie

Joseana Severo, Aline Tiecher, Jullien Pirrello, Farid Regad, Alain Latché, et al.. UV-C radiation modifies the ripening and accumulation of ethylene response factor (ERF) transcripts in tomato fruit. Postharvest Biology and Technology, 2015, 102, pp.9-16. ⟨10.1016/j.postharvbio.2015.02.001⟩. ⟨hal-01264327⟩