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The centrosome is an actin-organizing centre.

Abstract : Microtubules and actin filaments are the two main cytoskeleton networks supporting intracellular architecture and cell polarity. The centrosome nucleates and anchors microtubules and is therefore considered to be the main microtubule-organizing centre. However, recurring, yet unexplained, observations have pointed towards a connection between the centrosome and actin filaments. Here we have used isolated centrosomes to demonstrate that the centrosome can directly promote actin-filament assembly. A cloud of centrosome-associated actin filaments could be identified in living cells as well. Actin-filament nucleation at the centrosome was mediated by the nucleation-promoting factor WASH in combination with the Arp2/3 complex. Pericentriolar material 1 (PCM1) seemed to modulate the centrosomal actin network by regulating Arp2/3 complex and WASH recruitment to the centrosome. Hence, our results reveal an additional facet of the centrosome as an intracellular organizer and provide mechanistic insights into how the centrosome can function as an actin-filament-organizing centre.
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https://hal.archives-ouvertes.fr/hal-01261646
Contributor : Jacqueline Martin-Laffon Connect in order to contact the contributor
Submitted on : Monday, January 25, 2016 - 4:27:06 PM
Last modification on : Tuesday, May 11, 2021 - 11:36:30 AM

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Francesca Farina, Jérémie Gaillard, Christophe Guérin, Yohann Couté, James Sillibourne, et al.. The centrosome is an actin-organizing centre.. Nature Cell Biology, 2016, 18 (1), pp.65-75. ⟨10.1038/ncb3285⟩. ⟨hal-01261646⟩

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