Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes

Emilie Cardona (1, 2) , Bénédicte Lorgeoux (2) , Claire Geoffroy (1) , Pierre Richard (3) , Denis Saulnier (2) , Yannick Gueguen (4) , Gaël Guillou (3) , Liet Chim (1)

1 LEADNC - Unité Lagons, Ecosystèmes et Aquaculture Durable en Nouvelle-Calédonie
2 COP - Centre Océanologique du Pacifique
3 LIENSs - LIttoral ENvironnement et Sociétés
4 IHPE - Interactions Hôtes-Pathogènes-Environnements

The aim of this study was to assess the relative contribution of natural productivity and compound food to the growth of the juvenile blue shrimp Litopenaeus stylirostris reared in a biofloc system. Two experiments were carried out based on the sameprotocol with three treatments: clear waterwith experimental diet (CW), biofloc with experimental diet (BF) and biofloc unfed (BU). Shrimp survivalwas significantly higher in biofloc rearing than in CW rearing. The contribution of the biofloc to shrimp diet was estimated through measurement of carbon and nitrogen stable isotope ratios in shrimp and food sources. Different isotopic compositions between feeds were obtained by feeding natural productivitywith a mixture rich in fish meal and the shrimpswith a pellet containing a high level of soy protein concentrate. Using a two source one-isotope mixing model, we found that the natural productivity of the biofloc systemcontributed to shrimp growth at a level of 39.8% and 36.9%, for C and N, respectively. The natural food consumed by the shrimps reared in the biofloc system resulted in higher gene expression (mRNA transcript abundance) and activities of two digestive enzymes in their digestive gland: α-amylase and trypsin. The growth of shrimp biomass reared in biofloc was, on average, 4.4 times that of those grown in clear water. Our results confirmed the best survival and promoted growth of shrimps using biofloc technology and highlighted the key role of the biofloc in the nutrition of rearing shrimps.

Mots clés: Biofloc; Shrimps; Stable isotope; Digestive enzyme activities; Digestive enzyme mRNA level

Domaines: Evolution [q-bio.PE]

Emilie Cardona, Bénédicte Lorgeoux, Claire Geoffroy, Pierre Richard, Denis Saulnier, et al.. Relative contribution of natural productivity and compound feed to tissue growth in blue shrimp (Litopenaeus stylirostris) reared in biofloc: Assessment by C and N stable isotope ratios and effect on key digestive enzymes. Aquaculture, 2015, 448, pp.288-297. ⟨10.1016/j.aquaculture.2015.05.035⟩. ⟨hal-01259901⟩