CORRECTION OF PHOTOBLEACHING FOR THE ASSESSMENT OF PHARMACOKINETIC PARAMETERS USING DYNAMIC FLUORESCENCE MICROSCOPY

Marc Derieppe (1) , Clemens Bos (1) , M. de Greef (1) , Chrit Moonen (1) , Baudouin Denis de Senneville (1, 2)

1 University Medical Center [Utrecht]
2 IMB - Institut de Mathématiques de Bordeaux

Local drug delivery in oncology aims at depositing high doses of anticancer agents while limiting their toxic side effects. Biological barriers, such as cell plasma membranes, hinder their delivery and requires strategies to address this challenge. Previously [1], we demonstrated the feasibility to monitor in real-time, with dynamic fluorescence microscopy, the in-tracellular delivery of a hydrophilic model drug mediated by ultrasound (US), and to quantify the pharmacokinetic parameters derived from a two-compartment model. We evaluate here the impact of the photobleaching (PB) effect experimentally , and compute the PB-corrected uptake kinetics.

Domaines: Traitement du signal et de l'image [eess.SP]

Marc Derieppe, Clemens Bos, M. de Greef, Chrit Moonen, Baudouin Denis de Senneville. CORRECTION OF PHOTOBLEACHING FOR THE ASSESSMENT OF PHARMACOKINETIC PARAMETERS USING DYNAMIC FLUORESCENCE MICROSCOPY. IEEE International Symposium on Biomedical Imaging (ISBI), May 2015, New-York, United States. ⟨hal-01259351⟩