A New High-Throughput Approach to Genotype Ancient Human Gastrointestinal Parasites

Abstract : Human gastrointestinal parasites are good indicators for hygienic conditions and health status of past and present individuals and communities. While microscopic analysis of eggs in sediments of archeological sites often allows their taxonomic identification, this method is rarely effective at the species level, and requires both the survival of intact eggs and their proper identification. Genotyping via PCR-based approaches has the potential to achieve a precise species-level taxonomic determination. However, so far it has mostly been applied to individual eggs isolated from archeological samples. To increase the throughput and tax-onomic accuracy, as well as reduce costs of genotyping methods, we adapted a PCR-based approach coupled with next-generation sequencing to perform precise taxonomic identification of parasitic helminths directly from archeological sediments. Our study of twenty-five 100 to 7,200 year-old archeological samples proved this to be a powerful, reliable and efficient approach for species determination even in the absence of preserved eggs, either as a stand-alone method or as a complement to microscopic studies.
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Submitted on : Tuesday, January 19, 2016 - 2:33:27 PM
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Nathalie M.-L. Côte, Julien Daligault, Mélanie Pruvost, Olivier Gorgé, Silvia Guimaraes, et al.. A New High-Throughput Approach to Genotype Ancient Human Gastrointestinal Parasites. PLoS ONE, Public Library of Science, 2016, 11 (1), pp.e0146230. ⟨10.1371/journal.pone.0146230⟩. ⟨hal-01258207⟩



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