Potential of online comprehensive two-dimensional liquid chromatography for micro-preparative separations of simple samples

Abstract : Generally online comprehensive two-dimensional liquid chromatography (LC × LC) is used at preparative scale to prepare pure compounds from very complex samples which cannot be separated by one-dimensional liquid chromatography (1D-LC), even at analytical scale. In this work, micro-preparative online LC × LC was evaluated for the recovery of minor components from a simple mixture which can be separated by 1D-LC. This study is a proof of concept made from a test sample of four compounds which were separated by two 1D-LC methods and an LC × LC method based on the previous 1D-LC ones. The comparison of the preparative performance of the three methods shows that online LC × LC was able to achieve a 12-fold gain in amount recovered per injection compared to 1D-LC.
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https://hal.archives-ouvertes.fr/hal-01255464
Contributor : Agnès Bussy <>
Submitted on : Wednesday, January 13, 2016 - 5:05:09 PM
Last modification on : Thursday, February 8, 2018 - 11:10:27 AM

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Amélie Corgier, Morgan Sarrut, Gérard Crétier, Sabine Heinisch. Potential of online comprehensive two-dimensional liquid chromatography for micro-preparative separations of simple samples. Chromatographia, Springer Verlag, 2016, 79 (3), pp.225-260. ⟨10.1007/s10337-015-3012-x⟩. ⟨hal-01255464⟩

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