Ex vivo multiscale quantitation of skin biomechanics in wild-type and genetically-modified mice using multiphoton microscopy

Abstract : Soft connective tissues such as skin, tendon or cornea are made of about 90% of extracellular matrix proteins, fibrillar collagens being the major components. Decreased or aberrant collagen synthesis generally results in defective tissue mechanical properties as the classic form of Elhers-Danlos syndrome (cEDS). This connective tissue disorder is caused by mutations in collagen V genes and is mainly characterized by skin hyperextensibility. To investigate the relationship between the microstructure of normal and diseased skins and their macroscopic mechanical properties, we imaged and quantified the microstructure of dermis of ex vivo murine skin biopsies during uniaxial mechanical assay using multiphoton microscopy. We used two genetically-modified mouse lines for collagen V: a mouse model for cEDS harboring a Col5a2 deletion (a.k.a. pN allele) and the transgenic K14-COL5A1 mice which overexpress the human COL5A1 gene in skin. We showed that in normal skin, the collagen fibers continuously align with stretch, generating the observed increase in mechanical stress. Moreover, dermis from both transgenic lines exhibited altered collagen reorganization upon traction, which could be linked to microstructural modifications. These findings show that our multiscale approach provides new crucial information on the biomechanics of dermis that can be extended to all collagen-rich soft tissues.
Liste complète des métadonnées

Littérature citée [54 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01252023
Contributeur : Petr Dokladal <>
Soumis le : mardi 2 février 2016 - 10:34:40
Dernière modification le : vendredi 27 octobre 2017 - 17:36:02
Document(s) archivé(s) le : samedi 12 novembre 2016 - 01:38:00

Fichier

srep17635.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Licence


Distributed under a Creative Commons Paternité 4.0 International License

Identifiants

Citation

Stéphane Bancelin, Barbara Lynch, Christelle Bonod-Bidaud, Guillaume Ducourthial, Sotiris Psilodimitrakopoulos, et al.. Ex vivo multiscale quantitation of skin biomechanics in wild-type and genetically-modified mice using multiphoton microscopy. Scientific Reports, Nature Publishing Group, 2015, 5, pp.17635 〈http://www.nature.com/articles/srep17635〉. 〈10.1038/srep17635〉. 〈hal-01252023〉

Partager

Métriques

Consultations de la notice

478

Téléchargements de fichiers

52