Skip to Main content Skip to Navigation
Journal articles

Combining 'dry' co-crystallization and in situ diffraction to facilitate ligand screening by X-ray crystallography.

Abstract : X-ray crystallography is an established technique for ligand screening in fragment-based drug-design projects, but the required manual handling steps - soaking crystals with ligand and the subsequent harvesting - are tedious and limit the throughput of the process. Here, an alternative approach is reported: crystallization plates are pre-coated with potential binders prior to protein crystallization and X-ray diffraction is performed directly 'in situ' (or in-plate). Its performance is demonstrated on distinct and relevant therapeutic targets currently being studied for ligand screening by X-ray crystallography using either a bending-magnet beamline or a rotating-anode generator. The possibility of using DMSO stock solutions of the ligands to be coated opens up a route to screening most chemical libraries.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01234554
Contributor : Frank Thomas <>
Submitted on : Friday, November 27, 2015 - 8:29:15 AM
Last modification on : Wednesday, October 14, 2020 - 4:17:21 AM

Links full text

Identifiers

Collections

Citation

Muriel Gelin, Vanessa Delfosse, Frédéric Allemand, François Hoh, Yoann Sallaz-Damaz, et al.. Combining 'dry' co-crystallization and in situ diffraction to facilitate ligand screening by X-ray crystallography.. Acta Crystallographica Section D: Biological Crystallography, International Union of Crystallography, 2015, 71 (Pt 8), pp.1777-87. ⟨10.1107/S1399004715010342⟩. ⟨hal-01234554⟩

Share

Metrics

Record views

349