Cytotoxicity on Allium cepa of the two main sulcotrione photoproducts, xanthene-1,9-dione-3,4-dihydro-6-methylsulphonyl and 2-chloro-4-mesylbenzoic acid

Eric Goujon (1) , Claire Richard (2) , Pascale Goupil (1) , Gérard Ledoigt (1)

1 PIAF - Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier
2 ICCF - Institut de Chimie de Clermont-Ferrand

The cytotoxic effects of 2-chloro-4-mesylbenzoic acid (CMBA) and xanthene-1,9-dione-3,4-dihydro-6-methylsulphonyl (XDD), the two main photoproducts of sulcotrione, were investigated on Allium root meristematic cells at different concentrations. Degradation of sulcotrione was correlated to mitotic index decrease, together with increasing anomaly and c-mitosis frequencies. Mitotic index significantly decreased with increasing XDD and CMBA concentrations. Cell frequency with abnormal chromosomes increased with CMBA or XDD application rates. In contrast, CMBA induced a low micronucleus rate even for high concentrations while XDD increased the micronucleus ratio. C-mitoses, chromosomal aberrations due to an inactivation of the spindle, were enhanced by CMBA treatments but not by XDD. The photochemical degradation process of the pesticide can change the risk for the environment. (C) 2015 Elsevier Inc. All rights reserved.

Mots clés: Allium cepa; Pesticide; Photolysis; induced oxidative stress; 4-hydroxyphenylpyruvate dioxygenase; herbicide; sulcotrione; bleaching herbicide; vibrio-fischeri; degradation; soil; photodegradation; genotoxicity; inhibition

Domaines: Biologie végétale

Eric Goujon, Claire Richard, Pascale Goupil, Gérard Ledoigt. Cytotoxicity on Allium cepa of the two main sulcotrione photoproducts, xanthene-1,9-dione-3,4-dihydro-6-methylsulphonyl and 2-chloro-4-mesylbenzoic acid. Pesticide Biochemistry and Physiology, 2015, 124, pp.37-42. ⟨10.1016/j.pestbp.2015.04.001⟩. ⟨hal-01228848⟩