Skip to Main content Skip to Navigation
Journal articles

Malaria Sporozoites Traverse Host Cells within Transient Vacuoles

Abstract : Plasmodium sporozoites are deposited in the host skin by Anopheles mosquitoes. The parasites migrate from the dermis to the liver, where they invade hepatocytes through a moving junction (MJ) to form a replicative parasitophorous vacuole (PV). Malaria sporozoites need to traverse cells during progression through host tissues, a process requiring parasite perforin-like protein 1 (PLP1). We find that sporozoites traverse cells inside transient vacuoles that precede PV formation. Sporozoites initially invade cells inside transient vacuoles by an active MJ-independent process that does not require vacuole membrane remodeling or release of parasite secretory organelles typically involved in invasion. Sporozoites use pH sensing and PLP1 to exit these vacuoles and avoid degradation by host lysosomes. Next, parasites enter the MJ-dependent PV, which has a different membrane composition, precluding lysosome fusion. The malaria parasite has thus evolved different strategies to evade host cell defense and establish an intracellular niche for replication.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://hal.sorbonne-universite.fr/hal-01226222
Contributor : Gestionnaire Hal-Upmc <>
Submitted on : Monday, November 9, 2015 - 10:13:52 AM
Last modification on : Saturday, April 11, 2020 - 2:01:42 AM
Long-term archiving on: : Wednesday, February 10, 2016 - 10:14:26 AM

Identifiers

Citation

Veronica Risco-Castillo, Selma Topçu, Carine Marinach, Giulia Manzoni, Amélie e. Bigorgne, et al.. Malaria Sporozoites Traverse Host Cells within Transient Vacuoles. Cell Host and Microbe, Elsevier, 2015, 18 (51), pp.593-603. ⟨10.1016/j.chom.2015.10.006⟩. ⟨hal-01226222⟩

Share

Metrics

Record views

490

Files downloads

1076