XAS examination of glutathione cobalt complexes in solution.

Abstract : In the present work, we have investigated the coordination modes of cobalt with glutathione (γ-l-glutamyl-l-cysteinyl-glycine, GSH). A systematic study of cobalt-GSH complexes at basic and neutral pH has been undertaken with a multi-spectroscopic approach combined with quantum chemistry calculations. XAS (x-ray absorption spectroscopy) has been performed at the cobalt K edge in order to shed light into the cation coordination sphere and formal oxidation states. XANES (x-ray absorption near edge structure) enabled to show that in basic and neutral media, cobalt oxidation state is equal to +III and +II respectively. EXAFS (extended x-ray absorption fine structure) provided indications on the donor atoms involved in the coordination with cobalt as well as the bond lengths. DFT (density functional theory)-based calculations and NMR experiments have been performed to assess the most stable structure of the cobalt-GSH complex in basic conditions.
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Contributor : Riccardo Spezia <>
Submitted on : Thursday, October 22, 2015 - 5:50:54 PM
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Carole Bresson, Riccardo Spezia, Pier Lorenzo Solari, C.K. Jankowski, Christope den Auwer. XAS examination of glutathione cobalt complexes in solution.. Journal of Inorganic Biochemistry, Elsevier, 2015, 142, pp.126. ⟨10.1016/j.jinorgbio.2014.10.006⟩. ⟨hal-01219476⟩



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