Galactose Oxidase/Prussian Blue Based Biosensors.

Abstract : This paper describes the development of an amperometric biosensor based on galactose oxidase (GAOx) immobilization within a laponite clay film deposited on Carbon Screen-Printed Electrodes modified by electrodeposited Prussian Blue and coated with poly-(O-phenylenediamine) (PPD/PB/CSPEs). Amperometric performances of GAOx@laponite/PPD/PB/CSPEs bioelectrodes were determined using several GAOx substrates. Using these modified electrodes the reduction of enzymatically generated hydrogen peroxide was performed at −0.2 V vs. Ag-AgCl. In an initial attempt, E.Coli transketolase activity on its immobilized form was followed using a bienzymatic GAOx-TK biosensor.
Type de document :
Article dans une revue
Electroanalysis, Wiley-VCH Verlag, 2015, 27, pp.1341-1344. 〈10.1002/elan.201400720〉
Domaine :
Liste complète des métadonnées
Contributeur : Stéphanie Bonnefoy <>
Soumis le : jeudi 8 octobre 2015 - 14:44:20
Dernière modification le : mardi 19 mars 2019 - 01:24:01

Lien texte intégral



Franck Charmantray, Nadia Touisni, Laurence Hecquet, Thierry Noguer, Christine Mousty. Galactose Oxidase/Prussian Blue Based Biosensors.. Electroanalysis, Wiley-VCH Verlag, 2015, 27, pp.1341-1344. 〈10.1002/elan.201400720〉. 〈hal-01213418〉



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