Abstract : This study reports on the relationship between polybrominated diphenyl ether (PBDE) levels in water, sediment, and the benthic macroinvertebrate Gammarus pulex, which plays a major ecological role in freshwater ecosystems. Samples were taken in a periurban watershed (near Paris, France), and PBDEs were systematically detected in sediment (a parts per thousand currency sign727 ng g(-1) OC) and G. pulex (a parts per thousand currency sign264 ng g(-1) lipids). PBDEs were also occasionally detected in the water column at low levels (a PBDEs < 1.5 ng L-1). The log values of bioaccumulation factors were in the range 7.8 +/- A 0.1-8.3 +/- A 0.4 L kg(-1) for tetra- and penta-BDEs, which were the only ones quantified in the dissolved phase of river water. Meanwhile, levels of individual tri- to hepta-PBDE congeners in G. pulex generally positively correlated with sediment levels, suggesting an equilibrium situation. Biota-to-sediment accumulation factors (BSAFs) of tri-hepta BDEs were congener specific and were in the range 0.5 +/- A 0.3-2.6 +/- A 1.2. For several PBDEs, BSAF values deviated from the expected range, likely because of in vivo metabolism.