The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3

Liudmila Chelysheva (1) , Daniel Vezon (1) , Aurélie Chambon (1) , Ghislaine Gendrot (2) , Lucie Pereira (1) , Afef Lemhamdi (1) , Nathalie Vrielynck (1) , Sylvia Le Guin (1) , Maria Novatchkova (3, 4) , Mathilde Grelon (1)

1 IJPB - Institut Jean-Pierre Bourgin
2 RDP - Reproduction et développement des plantes
3 IMP - Research Institute of Molecular Pathology
4 Inst Mol Biotechnol

In numerous species, the formation of meiotic crossovers is largely under the control of a group of proteins known as ZMM. Here, we identified a new ZMM protein, HEI10, a RING finger-containing protein that is well conserved among species. We show that HEI10 is structurally and functionally related to the yeast Zip3 ZMM and that it is absolutely required for class I crossover (CO) formation in Arabidopsis thaliana. Furthermore, we show that it is present as numerous foci on the chromosome axes and the synaptonemal complex central element until pachytene. Then, from pachytene to diakinesis, HEI10 is retained at a limited number of sites that correspond to class I COs, where it co-localises with MLH1. Assuming that HEI10 early staining represents an early selection of recombination intermediates to be channelled into the ZMM pathway, HEI10 would therefore draw a continuity between early chosen recombination intermediates and final class I COs.

Mots clés: STRAND-EXCHANGE; MISMATCH REPAIR; MAMMALIAN MEIOSIS; RECOMBINATION NODULES; SACCHAROMYCES-CEREVISIAE; CROSSING-OVER; CHROMOSOME SYNAPSIS; SYNAPTONEMAL COMPLEX; MEIOTIC CROSSOVER FORMATION; DNA BREAK REPAIR

Domaines: Sciences du Vivant [q-bio]

Liudmila Chelysheva, Daniel Vezon, Aurélie Chambon, Ghislaine Gendrot, Lucie Pereira, et al.. The Arabidopsis HEI10 Is a New ZMM Protein Related to Zip3. PLoS Genetics, 2012, 8 (7), ⟨10.1371/journal.pgen.1002799⟩. ⟨hal-01190765⟩