Feasibility of application of conductometric biosensor based on acetylcholinesterase for the inhibitory analysis of toxic compounds of different nature

K. V. Stepurska (1, 2, 3) , O. O. Soldatkin (1, 2) , I. S. Kucherenko (1, 3) , V. M. Arkhypova (1, 2) , S.V. Dzyadevych (1, 2)

1 Lab Biomol Elect
2 Institute of High Technologies [Kyiv]
3 ISA-Interfaces & biosensors - Interfaces & biocapteurs

This study was aimed at the development of a conductometric biosensor based on acetylcholinesterase considering the feasibility of its application for the inhibitory analysis of various toxicants. In this paper, the optimum conditions for enzyme immobilization on the transducer surface are selected as well as the optimum concentration of substrate for inhibitory analysis. Sensitivity of the developed biosensor to different classes of toxic compounds (organophosphorus pesticides, heavy metal ions, surfactants, aflatoxin, glycoalkaloids) was tested. It is shown that the developed biosensor can be successfully used for the analysis of pesticides and mycotoxins, as well as for determination of total toxicity of the samples. A new method of biosensor analysis of toxic substances of different classes in complex multicomponent aqueous samples is proposed.

Mots clés: GLYCOALKALOIDS; AFLATOXIN-B1; ISFETS; Toxic compounds; Inhibitory analysis; Conductometric biosensor; Acetylcholinesterase; CHOLINESTERASES

Domaines: Chimie analytique

K. V. Stepurska, O. O. Soldatkin, I. S. Kucherenko, V. M. Arkhypova, S.V. Dzyadevych. Feasibility of application of conductometric biosensor based on acetylcholinesterase for the inhibitory analysis of toxic compounds of different nature. Analytica Chimica Acta, 2015, 854, pp.161-168. ⟨10.1016/j.aca.2014.11.027⟩. ⟨hal-01187433⟩