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Two different and functional nuclear rDNA genes in the abalone Haliotis tuberculata : tissue differential expression

Alain van Wormhoudt 1 Béatrice Gaume 2 Yvan Le Bras 3 Valérie Roussel 1 Sylvain Huchette 4
1 Station de Biologie Marine de Concarneau
DGD.REVE - Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation
3 Plateforme bioinformatique GenOuest [Rennes]
UR1 - Université de Rennes 1, Plateforme Génomique Santé Biogenouest®, Inria Rennes – Bretagne Atlantique , IRISA-D7 - GESTION DES DONNÉES ET DE LA CONNAISSANCE
Abstract : Analysis of the 18S rDNA sequences of Haliotis tuberculata tuberculata and H. t. coccinea subtaxa identified two different types of 18S rDNA genes and ITS1 regions. These two different genes were also detected in H. marmorata, H. rugosa and H. diversicolor that are separated from H. tuberculata by 5–65 mya. The mean divergence value between type I and type II sequences ranged from 7.25% for 18S to 80% for ITS1. ITS1 type II is homologous with the ITS1 consensus sequences published for many abalone species, whereas ITS1 type I presented only minor homology with a unique database entry for H. iris ITS1. A phylogenetic analysis makes a clear separation between type I and type II ITS1 sequences and supports grouping H. t. tuberculata, H. t. coccinea and H. marmorata together. The two subtaxa do not show any significant differences between the homologous 18S rDNA sequences. A general structure of the ITS1 transcript was proposed, with four major helices for the two types. The two genes were expressed and, for the first time, a putative differential expression of ITS1 type I was detected in the gills, digestive gland and gonads whereas ITS1 type II was expressed in all tissues.
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https://hal.archives-ouvertes.fr/hal-01183596
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Submitted on : Monday, August 10, 2015 - 11:58:33 AM
Last modification on : Friday, July 10, 2020 - 4:23:54 PM

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Alain van Wormhoudt, Béatrice Gaume, Yvan Le Bras, Valérie Roussel, Sylvain Huchette. Two different and functional nuclear rDNA genes in the abalone Haliotis tuberculata : tissue differential expression. Genetica, Springer Verlag, 2011, http://link.springer.com/article/10.1007%2Fs10709-011-9623-8. ⟨10.1007/s10709-011-9623-8⟩. ⟨hal-01183596⟩

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