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Yeast prions assembly and propagation: contributions of the prion and non-prion moieties and the nature of assemblies.

Abstract : Yeast prions are self-perpetuating protein aggregates that are at the origin of heritable and transmissible non-Mendelian phenotypic traits. Among these, [PSI+], [URE3] and [PIN+] are the most well documented prions and arise from the assembly of Sup35p, Ure2p and Rnq1p, respectively, into insoluble fibrillar assemblies. Fibril assembly depends on the presence of N- or C-terminal prion domains (PrDs) which are not homologous in sequence but share unusual amino-acid compositions, such as enrichment in polar residues (glutamines and asparagines) or the presence of oligopeptide repeats. Purified PrDs form amyloid fibrils that can convert prion-free cells to the prion state upon transformation. Nonetheless, isolated PrDs and full-length prion proteins have different aggregation, structural and infectious properties. In addition, mutations in the "non-prion" domains (non-PrDs) of Sup35p, Ure2p and Rnq1p were shown to affect their prion properties in vitro and in vivo. Despite these evidences, the implication of the functional non-PrDs in fibril assembly and prion propagation has been mostly overlooked. In this review, we discuss the contribution of non-PrDs to prion assemblies, and the structure-function relationship in prion infectivity in the light of recent findings on Sup35p and Ure2p assembly into infectious fibrils from our laboratory and others.
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https://hal.archives-ouvertes.fr/hal-01183179
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Submitted on : Thursday, August 6, 2015 - 3:30:05 PM
Last modification on : Thursday, November 5, 2020 - 10:46:07 AM

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Mehdi Kabani, Ronald Melki. Yeast prions assembly and propagation: contributions of the prion and non-prion moieties and the nature of assemblies.. Prion, Taylor & Francis, 2011, 5 (4), pp.277-84. ⟨10.4161/pri.18070⟩. ⟨hal-01183179⟩

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