Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays

Abstract : Droplet-based microfluidic technologies are powerful tools for applications requiring high-throughput, for example, in biochemistry or material sciences. Several systems have been proposed for the high-throughput production of monodisperse emulsions by parallelizing multiple droplet makers. However, these systems have two main limitations: (1) they allow the use of only a single disperse phase; (2) they are based on multiple layer microfabrication techniques. We present here a pipette-and-play solution offering the possibility of manipulating simultaneously 10 different disperse phases on a single layer device. This system allows high-throughput emulsion production using aqueous flow rates of up to 26 ml/h (>110 000 drops/s) leading to emulsions with user-defined complex chemical composition. We demonstrate the multiplex capabilities of our system by measuring the kinetics of b-galactosidase in droplets using nine different concentrations of a fluorogenic substrate.
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Jiseok Lim, Ouriel Caen, Jérémy Vrignon, Manfred Konrad, Valérie Taly, et al.. Parallelized ultra-high throughput microfluidic emulsifier for multiplex kinetic assays. Biomicrofluidics, 2015, 9, pp.034101 (1-11). ⟨10.1063/1.4919415⟩. ⟨hal-01179083⟩

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