Genomic characterization of EmsB microsatellite loci in Echinococcus multilocularis.

Benoît Valot (1) , Jenny Knapp (2, 3) , Gérald Umhang (4) , Frédéric Grenouillet (3, 2, 5) , Laurence Millon (5, 3, 2)

1 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)
2 WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis
3 LCE - Laboratoire Chrono-environnement (UMR 6249)
4 ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail
5 Service de parasitologie et mycologie [CHRU de Besançon]

EmsB is a molecular marker applied to Echinococcus multilocularis genotyping studies. This marker has largely been used to investigate the epidemiology of the parasite in different endemic foci. The present study has lifted the veil on the genetic structure of this microsatellite. By in silico analysis on the E. multilocularis genome the microsatellite was described in about 40 copies on the chromosome 5 of the parasite. Similar structure was found in the relative parasite Echinococcus granulosus, where the microsatellite was firstly described. The present study completes the first investigations made on the EmsB microsatellite origins and confirms the reliability of this highly discriminant molecular marker.

Mots clés: EmsB microsatellite; Echinococcus multilocularis; Genetic diversity; Genome; In silico analysis

Domaines: Mycologie

Benoît Valot, Jenny Knapp, Gérald Umhang, Frédéric Grenouillet, Laurence Millon. Genomic characterization of EmsB microsatellite loci in Echinococcus multilocularis.. Infection, Genetics and Evolution, 2015, 32, pp.338-41. ⟨10.1016/j.meegid.2015.03.040⟩. ⟨hal-01166207⟩