Skip to Main content Skip to Navigation
Journal articles

Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.

Abstract : In this study we demonstrate the use of adaptive optics to correct the biasing effects of optical aberrations when measuring the dynamics of molecules diffusing between cells in multicellular spheroids. Our results indicate that, on average, adaptive optics leads to a reduction of the 3D size of the point spread function that is statistically significant in terms of measured number of molecules and diffusion time. The sensorless approach, which uses the molecular brightness as optimization metric, is validated in a complex, highly heterogeneous, biological environment. This work paves the way towards the design of accurate diffusion measurements of molecules in thick biological specimens.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-01123925
Contributor : Giovanni Cappello <>
Submitted on : Thursday, March 5, 2015 - 5:32:29 PM
Last modification on : Tuesday, October 6, 2020 - 4:24:13 PM

Links full text

Identifiers

Citation

Charles-Edouard Leroux, Sylvain Monnier, Irène Wang, Giovanni Cappello, Antoine Delon. Fluorescent correlation spectroscopy measurements with adaptive optics in the intercellular space of spheroids.. Biomedical optics express, Optical Society of America - OSA Publishing, 2014, 5 (10), pp.3730-8. ⟨hal-01123925⟩

Share

Metrics

Record views

335