Synthesis and characterization of multimodal Gd-Nanoparticles vectorized for Amyloidosis diagnosis, and their affinity evaluation using Biacore.
Résumé
Amyloidosis is a systemic disorder in which proteins form insoluble aggregates (or fibrils) in extracellular tissues. Depending of the protein nature and its deposition area, this accumulation could lead to different pathologies. In this project, we focus on three kind of amyloidosis forms: i) Amyloid Aβ, ii) Islet Amyloid PolyPeptide IAPP, iii) Transthyretin TTR, which are respectively responsible of Alzheimer Disease, type II diabetes and finely cardiomyopathy and polyneuropathy.
We are investigating two different strategies to target the fibrils: i) Camelid heavy chain antibody fragment (or Nanobody)[1], ii) Peptides derived from the protein responsible of the aggregate.