A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting

Ambroise Marin (1) , Emmanuel Denimal (1) , Stéphane Guyot (2) , Ludovic Journaux (3) , Paul Molin (2)

1 Service d’Appui à la Recherche - AgroSup Dijon
2 PMB - Procédés Microbiologiques et Biotechnologiques
3 Le2i - Laboratoire Electronique, Informatique et Image [UMR6306]

In biology, cell counting is a primary measurement and it is usually performed manually using hemocytometers such as Malassez blades. This work is tedious and can be automated using image processing. An algorithm based on Fourier transform filtering and the Hough transform was developed for Malassez blade grid extraction. This facilitates cell segmentation and counting within the grid. For the present work, a set of 137 images with high variability was processed. Grids were accurately detected in 98% of these images.

Mots clés: morphological mathematics; cell segmentation; image analysis; Hough transform; Malassez; Fourier transform

Domaines: Traitement des images [eess.IV]

Ambroise Marin, Emmanuel Denimal, Stéphane Guyot, Ludovic Journaux, Paul Molin. A Robust Generic Method for Grid Detection in White Light Microscopy Malassez Blade Images in the Context of Cell Counting. Microscopy and Microanalysis, 2015, 21 (01), pp.239-248. ⟨10.1017/S1431927614013671⟩. ⟨hal-01114543⟩