Force Measurements of TCR/pMHC Recognition at T Cell Surface
Résumé
The rupture forces and adhesion frequencies of single recognition complexes between an affinity
selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using
Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends on
the nature of the peptide but the rupture force does not. When CD8 is present, no effect of the nature
of the peptide is observed. CD8 is proposed to act as a time and distance lock, enabling the shorter
TCR molecule to bridge the pMHC and have time to finely read the peptide. Ultimately, such
experiments could help the dissection of the sequential steps by which the TCR reads the
peptide/MHC complex in order to control T cell activation.
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