Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems?

Abstract : The nucleic acid contents of individual bacterial cells as determined with three different nucleic acid-specific fluorescent dyes (SYBR I, SYBR II, and SYTO 13) and flow cytometry were compared for different seawater samples. Similar fluorescence patterns were observed, and bacteria with high apparent nucleic acid contents (HNA) could be discriminated from bacteria with low nucleic acid contents (LNA). The best discrimination between HNA and LNA cells was found when cells were stained with SYBR II. Bacteria in different water samples collected from seven freshwater, brackish water, and seawater ecosystems were prelabeled with tritiated leucine and then stained with SYBR II. After labeling and staining, HNA, LNA, and total cells were sorted by flow cytometry, and the specific activity of each cellular category was determined from leucine incorporation rates. The HNA cells were responsible for most of the total bacterial production, and the specific activities of cells in the HNA population varied between samples by a factor of seven. We suggest that nucleic acid content alone can be a better indicator of the fraction of growing cells than total counts and that this approach should be combined with other fluorescent physiological probes to improve detection of the most active cells in aquatic systems.
Type de document :
Article dans une revue
Applied and Environmental Microbiology, American Society for Microbiology, 2001, 67, pp.1775 - 1782. 〈10.1128/AEM.67.4.1775–1782.2001〉
Liste complète des métadonnées

Littérature citée [27 références]  Voir  Masquer  Télécharger

https://hal.archives-ouvertes.fr/hal-01102845
Contributeur : Hélène Agogué <>
Soumis le : mardi 13 janvier 2015 - 15:50:19
Dernière modification le : lundi 17 décembre 2018 - 01:28:27
Document(s) archivé(s) le : mardi 14 avril 2015 - 11:15:28

Fichier

Lebaronetal, AEM.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Citation

Philippe Lebaron, Pierre Servais, Helene Agogué, Claude Courties, Fabien Joux. Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems?. Applied and Environmental Microbiology, American Society for Microbiology, 2001, 67, pp.1775 - 1782. 〈10.1128/AEM.67.4.1775–1782.2001〉. 〈hal-01102845〉

Partager

Métriques

Consultations de la notice

156

Téléchargements de fichiers

155