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A MAP6-related protein is present in protozoa and is involved in flagellum motility.

Abstract : In vertebrates the microtubule-associated proteins MAP6 and MAP6d1 stabilize cold-resistant microtubules. Cilia and flagella have cold-stable microtubules but MAP6 proteins have not been identified in these organelles. Here, we describe TbSAXO as the first MAP6-related protein to be identified in a protozoan, Trypanosoma brucei. Using a heterologous expression system, we show that TbSAXO is a microtubule stabilizing protein. Furthermore we identify the domains of the protein responsible for microtubule binding and stabilizing and show that they share homologies with the microtubule-stabilizing Mn domains of the MAP6 proteins. We demonstrate, in the flagellated parasite, that TbSAXO is an axonemal protein that plays a role in flagellum motility. Lastly we provide evidence that TbSAXO belongs to a group of MAP6-related proteins (SAXO proteins) present only in ciliated or flagellated organisms ranging from protozoa to mammals. We discuss the potential roles of the SAXO proteins in cilia and flagella function.
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https://hal.archives-ouvertes.fr/hal-01101397
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Submitted on : Thursday, January 8, 2015 - 3:40:51 PM
Last modification on : Wednesday, February 10, 2021 - 12:30:02 PM

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  • HAL Id : hal-01101397, version 1
  • PUBMED : 22355359

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Denis Dacheux, Nicolas Landrein, Magali Thonnus, Guillaume Gilbert, Annelise Sahin, et al.. A MAP6-related protein is present in protozoa and is involved in flagellum motility.. PLoS ONE, Public Library of Science, 2011, 7(2), pp.e31344. ⟨hal-01101397⟩

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