A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping

Sajid S. Ali (1) , Angelique Gautier (1) , Marc Leconte (1) , Jerome Enjalbert (2) , Claude C. Pope de Vallavieille (1)

1 BIOGER - BIOlogie et GEstion des Risques en agriculture
2 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)

Puccinia striiformis f.sp. tritici (PST), an obligate fungal pathogen causing wheat yellow/stripe rust, a serious disease, has been used to understand the evolution of crop pathogen using molecular markers. However, numerous questions regarding its evolutionary history and recent migration routes still remains to be addressed, which need the genotyping of a large number of isolates, a process that is limited by both DNA extraction and genotyping methods. To address the two issues, we developed here a method for direct DNA extraction from infected leaves combined with optimized SSR multiplexing.

Mots clés: genotyping method; multiplex pcr genotyping; fungal pathogen; genotype; microsatellitte; rouille jaune du blé; Puccinia striiformis; diversité génétique

Domaines: Sciences agricoles

Sajid S. Ali, Angelique Gautier, Marc Leconte, Jerome Enjalbert, Claude C. Pope de Vallavieille. A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping. BMC Research Notes, 2011, 4 (240), pp.1-5. ⟨10.1186/1756-0500-4-240⟩. ⟨hal-01019765⟩