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Evidence for an internal and functional circadian clock in rat pituitary cells.

Abstract : In primary cultures of rat pituitary cells and in a pituitary sommatolactotroph cell line (GH4C1), endogenous core-clock- as well as hormone-genes such as prolactin displayed a rhythmic expression pattern, fitted by a sinusoidal equation in which the period value was close to the circadian one. This is consistent with the presence of a functional circadian oscillator in pituitary cells whose importance was ascertained in GH4C1 cell lines stably expressing a dominant negative mutant of BMAL1. In these cells, both endogenous core-clock- and prolactin-genes no more displayed a circadian pattern. Some genes we recently identified as mouse pituitary BMAL1-regulated genes in a DNA-microarray study, lost their circadian pattern in these cells, suggesting that BMAL1 controlled these genes locally in the pituitary. The intra-pituitary circadian oscillator could then play a role in the physiology of the gland that would not be seen anymore as a structure only driven by hypothalamic rhythmic control.
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Contributor : Corinne Dumonceaud <>
Submitted on : Wednesday, March 26, 2014 - 11:53:29 AM
Last modification on : Wednesday, November 25, 2020 - 3:12:19 AM




Denis Becquet, Bénédicte Boyer, Ramahefarizo Rasolonjanahary, Thierry Brue, Séverine Guillen, et al.. Evidence for an internal and functional circadian clock in rat pituitary cells.. Molecular and Cellular Endocrinology, Elsevier, 2014, 382 (2), pp.888-98. ⟨10.1016/j.mce.2013.11.004⟩. ⟨hal-00966197⟩



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