DNA directed immobilization glycocluster array: applications and perspectives.

Yann Chevolot (1) , Emmanuelle Laurenceau (1) , Magali Phaner-Goutorbe (1) , Virginie Monnier (1) , Eliane Souteyrand (1) , Albert Meyer (2) , Thomas Gehin (1) , Jean-Jacques Vasseur (2) , François Morvan (2)

1 INL - C&N - INL - Chimie et Nanobiotechnologies
2 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

The present review concerns the recent advances in DNA directed immobilization (DDI) based glycocluster array. The impact of glycan immobilization on subsequent interactions with protein is discussed and the consequent pros and cons of DDI-based glycocluster array are reviewed. Finally, application in the discovery of anti-pathogen molecules is illustrated by screening for galactose or fucose glycoclusters targeting two Pseudomonas aeruginosa virulence factors (PA-IL and PA-IIL).

Mots clés: protein; anti-pathogen; pseudomonas aeruginosa; DNA; glycocluster; glycan; immobilization

Domaines: Chimie organique; Chimie thérapeutique; Biotechnologies

Yann Chevolot, Emmanuelle Laurenceau, Magali Phaner-Goutorbe, Virginie Monnier, Eliane Souteyrand, et al.. DNA directed immobilization glycocluster array: applications and perspectives.. Current Opinion in Chemical Biology, 2014, 18, pp.46-54. ⟨10.1016/j.cbpa.2013.12.009⟩. ⟨hal-00952596⟩