Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

Abstract : Chlorophyll meters such as the SPAD-502 offer a simple, inexpensive and rapid method to estimate foliar chlorophyll content. However, values provided by SPAD-502 are unitless and require empirical calibrations between SPAD units and extracted chlorophyll values. * Leaves of 13 tree species from the tropical rain forest in French Guiana were sampled to select the most appropriate calibration model among the often-used linear, polynomial and exponential models, in addition to a novel homographic model that has a natural asymptote.* The homographic model best accurately predicted total chlorophyll content (μg cm−2) from SPAD units (R2 = 0.89). Interspecific differences in the homographic model parameters explain less than 7% of the variation in chlorophyll content in our data set. * The utility of the general homographic model for a variety of research and management applications clearly outweighs the slight loss of model accuracy due to the abandon of the species' effect.
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Submitted on : Friday, January 1, 2010 - 10:00:00 AM
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Sabrina Coste, Christopher Baraloto, Céline Leroy, Eric Marcon, Amélie Renaud, et al.. Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana. Annals of Forest Science, Springer Verlag/EDP Sciences, 2010, 67 (6), ⟨10.1051/forest/2010020⟩. ⟨hal-00883628⟩

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