Fluorescence-based bacterial bioreporter for specific detection of methyl halide emissions in the environment.

Muhammad Farhan Ul Haque (1) , Thierry Nadalig (1) , Françoise Bringel (1) , Hubert Schaller (2) , Stéphane Vuilleumier (1)

1 GMGM - Génétique moléculaire, génomique, microbiologie
2 IBMP - Institut de Biologie Moléculaire des Plantes

Methyl halides are volatile one-carbon compounds responsible for substantial depletion of stratospheric ozone. Among them, chloromethane (CH3Cl) is the most abundant halogenated hydrocarbon in the atmosphere. Global budgets of methyl halides in the environment are still poorly understood due to uncertainties in their natural sources, mainly from vegetation, and their sinks, which include chloromethane-degrading bacteria. A bacterial bioreporter for the detection of methyl halides was developed on the basis of detailed knowledge of the physiology and genetics of Methylobacterium extorquens CM4, an aerobic alphaproteobacterium which utilizes chloromethane as the sole source of carbon and energy. A plasmid construct with the promoter region of the chloromethane dehalogenase gene cmuA fused to a promotorless yellow fluorescent protein gene cassette resulted in specific methyl halide-dependent fluorescence when introduced into M. extorquens CM4. The bacterial whole-cell bioreporter allowed detection of methyl halides at femtomolar levels and quantification at concentrations above 10 pM (approximately 240 ppt). As shown for the model chloromethane-producing plant Arabidopsis thaliana in particular, the bioreporter may provide an attractive alternative to analytical chemical methods to screen for natural sources of methyl halide emissions.

Domaines: Interactions entre organismes

Muhammad Farhan Ul Haque, Thierry Nadalig, Françoise Bringel, Hubert Schaller, Stéphane Vuilleumier. Fluorescence-based bacterial bioreporter for specific detection of methyl halide emissions in the environment.. Applied and Environmental Microbiology, 2013, 79 (21), pp.6561-7. ⟨10.1128/AEM.01738-13⟩. ⟨hal-00880763⟩