Expression of Aromatase in Radial Glial Cells in the Brain of the Japanese Eel Provides Insight into the Evolution of the cyp191a Gene in Actinopterygians.

Abstract : The cyp19a1 gene that encodes aromatase, the only enzyme permitting conversion of C19 aromatizable androgens into estrogens, is present as a single copy in the genome of most vertebrate species, except in teleosts in which it has been duplicated. This study aimed at investigating the brain expression of a cyp19a1 gene expressed in both gonad and brain of Japanese eel, a basal teleost. By means of immunohistochemistry and in situ hybridization, we show that cyp19a1 is expressed only in radial glial cells of the brain and in pituitary cells. Treatments with salmon pituitary homogenates (female) or human chorionic gonadotrophin (male), known to turn on steroid production in immature eels, strongly stimulated cyp19a1 messenger and protein expression in radial glial cells and pituitary cells. Using double staining studies, we also showed that aromatase-expressing radial glial cells exhibit proliferative activity in both the brain and the pituitary. Altogether, these data indicate that brain and pituitary expression of Japanese eel cyp19a1 exhibits characteristics similar to those reported for the brain specific cyp19a1b gene in teleosts having duplicated cyp19a1 genes. This supports the hypothesis that, despite the fact that eels also underwent the teleost specific genome duplication, they have a single cyp19a1 expressed in both brain and gonad. Such data also suggest that the intriguing features of brain aromatase expression in teleost fishes were not gained after the whole genome duplication and may reflect properties of the cyp19a1 gene of ancestral Actinopterygians.
Document type :
Journal articles
Complete list of metadatas

Cited literature [89 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-00877375
Contributor : Morgane Le Corre <>
Submitted on : Monday, October 28, 2013 - 1:25:28 PM
Last modification on : Thursday, July 11, 2019 - 2:10:07 PM
Long-term archiving on : Wednesday, January 29, 2014 - 4:47:20 AM

File

Expression_of_Aromatase-accept...
Publisher files allowed on an open archive

Identifiers

Citation

Shan-Ru Jeng, Wen-Shiun Yueh, Yi-Ting Pen, Marie-Madeleine Guéguen, Jérémy Pasquier, et al.. Expression of Aromatase in Radial Glial Cells in the Brain of the Japanese Eel Provides Insight into the Evolution of the cyp191a Gene in Actinopterygians.. PLoS ONE, Public Library of Science, 2012, 7 (9), pp.e44750. ⟨10.1371/journal.pone.0044750⟩. ⟨hal-00877375⟩

Share

Metrics

Record views

443

Files downloads

660