Study of the transmembrane potential distribution of cell pairs in a microfluidic device using polymer obstacles to initiate electrofusion - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Physical Journal: Applied Physics Année : 2013

Study of the transmembrane potential distribution of cell pairs in a microfluidic device using polymer obstacles to initiate electrofusion

Résumé

In this paper, we investigate the different parameters that affect the distribution of the transmembrane potential of cells brought into contact before electrofusion in a miniaturized fluidic device. In particular, we discuss the deviation of the effective transmembrane voltage compared to the one predicted by Schwan’s law. The application of electric field pulses to biological cells induces a transmembrane potential which leads to cell permeabilization. Electrofusion occurs when several cells are brought into contact while they are electropermeabilized. Nevertheless, we show that in this case, the mutual presence of cells interferes on Schwan’s equation. Consequently, the transmembrane voltage at the cell contacting point is drastically reduced, which is not favorable for an electrofusion in smooth conditions, as the applied voltage needs to be increased to compensate this phenomenon. We show that the introduction of polymer obstacles reverses this trend, as the high electric field region is focused on the fusion zone. To confirm the theory we developed, quantitative biological experiments are presented in which murine melanoma cells were paired and fused in both conditions (with and without obstacles).

Dates et versions

hal-00812130 , version 1 (11-04-2013)

Identifiants

Citer

Feriel Sihem Hamdi, Olivier Français, Frédéric Subra, Wei Wang, Marion Woytasik, et al.. Study of the transmembrane potential distribution of cell pairs in a microfluidic device using polymer obstacles to initiate electrofusion. European Physical Journal: Applied Physics, 2013, 62 (01), pp.11202-11212. ⟨10.1051/epjap/2013120392⟩. ⟨hal-00812130⟩
239 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More