High-resolution and sensitivity through-bond correlations in ultra-fast magic angle spinning (MAS) solid-state NMR

Ivano Bertini (1, 2) , Lyndon Emsley (3) , Isabella C. Felli (1, 2) , Ségolène Laage (3) , Anne Lesage (3) , Jozef R. Lewandowski (3) , Alessandro Marchetti (3, 4) , Roberta Pierattelli (1, 2) , Guido Pintacuda (3)

1 Dept Chem Ugo Schiff
2 Magnet Resonance Ctr CERM
3 ISA - Centre de RMN à très hauts champs
4 SNS - Scuola Normale Superiore di Pisa

We introduce a new experiment, which makes use of Spin State Selective manipulations to perform sensitive and resolved through-bond correlations in organic and biological solids at high-fields and under ultra-fast MAS. The scheme is the shortest and most sensitive through-bond correlation method introduced so far in solids, yields resolved fingerprints of uniformly (13)C-labeled biomolecules, and constitutes a tool to highlight slight static structural disorder around crystallographically equivalent molecules in microcrystalline samples.

Mots clés: SELECTION; DISORDERED SOLIDS; CROSS-POLARIZATION; HETERONUCLEAR CORRELATION; ASSIGNMENT STRATEGIES; SPECTROSCOPY; PROTEINS; C-13; HOMONUCLEAR; ENHANCEMENT

Domaines: Chimie analytique

Ivano Bertini, Lyndon Emsley, Isabella C. Felli, Ségolène Laage, Anne Lesage, et al.. High-resolution and sensitivity through-bond correlations in ultra-fast magic angle spinning (MAS) solid-state NMR. Chemical Science, 2011, 2 (2), pp.345-348. ⟨10.1039/c0sc00397b⟩. ⟨hal-00809106⟩